The VLPs are easily collected from the culture medium, providing a workflow that is much less labor intensive than preparation of traditional membrane fractions (Figure 2).įigure 2. This enables these proteins to be displayed in their native context in a highly enriched nano-lipoparticle. MembranePro technology delivers overexpressed membrane proteins on virus-like particles (VLPs) that self-assemble and bud off the cell surface. All components work in concert to additively increase expression. The key features that bring about the superior protein yields of the Expi293 system are the enriched Expi293 Expression Medium, which supports high density culture of superior productivity Expi293F cells, and transient expression powered by the novel ExpiFectamine 293 transfection reagent and enhancers, which are designed to work specifically under high-density culture conditions. All yields are given as mg of protein per L of culture transfected (mg/L).Įxpi293 MembranePro Expression System provides superior yields of difficult to express membrane proteins Protein was harvested at day 5 to day 7 post-transfection. Expression was performed in 1ml, 30mL and 1 liter cultures. Expression of human erythropoietin (EPO), crypto and a human IgG in the FreeStyle 293 system (light columns) compared to expression in Expi293 (dark columns). We have observed expression levels of greater than 1 gram per liter of culture for some proteins (Figure 1).įigure 1. Typical Expi293 yields are 2- to 10-fold higher than other options. The Expi293 Expression system is a rapid, scalable system that delivers the highest protein yields of any current transient expression method. Rapid and ultra high-yield protein production in mammalian cells
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